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Then, 20 μL of antibody inventory (200 μg) was added to the pattern and incubated three h at 37 °C adopted by a second incubation overnight at 4 °C . The subsequent day, the conjugated nanoparticles had been centrifuged and washed twice with borate buffer 5 mM pH 9. Then, 25 mg of BSA was added to the solution. After 1 h incubation at room temperature under mild shaking, the sample was washed with borate buffer supplemented with Tween 20 and finally stored at 4 °C till additional use…

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