In addition, the road scanning requires extra powerful laser compared with the point-scanning Raman microscope system . One main factor results in highly delicate SERS-LFA is deploying NPs with stronger plasmonic coupling impact. For instance, nanostructures with “hot spots” had been reported to enhance SERS indicators, including plasmonic NPs with rough floor, coupled NPs with nanogaps, and multibranched nanostructures [42-44].

Reported effect sizes in the relevant research were…