Z-stack: A set of confocal images taken from the specimen so that the image area along the x- and y-axes remains the same but the distance from the objective (z-axis) is different for each image. As Confocal Live Cell Microscopy For all practical purposes in fluorescence microscopy, a lambda stack can be considered analogous to a time-lapse series or optical section z-stack gathered by a widefield deconvolution or confocal microscope. Olympus FV1000 Multiphoton Manual. Print this page. Click Depth below the XY acquire button to enable to Z-stack acquisition and click the acquisition button to start the scan. Andor XD Spinning Disk Confocal Microscope Manual; ZEN 2.3 includes new features and improvements for our next-generation ZEISS Axiocam microscope cameras, ZEISS LSM 800 confocal microscope, ZEISS Software Tutorial: Introduction to the ZEN 2.3 3Dxl Module Once I've acquired a Z-stack, I then click on the "ApoTome" tab to "create image Cell bio- 9. STUDY. PLAY. All plant and animal tissues are made up of cells. Cell theory (doctrine) confocal microscopy. resolution much better than conventional. confocal microscopy. has z stacking capacity, uses electrons that are scattered to make image, provides great depth of field. Advances in confocal microscopy have made possible multi-dimensional views of living cells and tissues that include image information in the x, y, and z dimensions as a function of time and presented in multiple colors (using two or more fluorophores). After volume processing of individual image stacks, the resulting data can be displayed as The tutorial initializes with a lambda stack of three nuclei expressing ECFP, EGFP, and EYFP appearing in the window. a lambda stack can be considered analogous to a time-lapse series or optical section z-stack gathered by a widefield deconvolution or confocal microscope. Biology Imaging Microscope Confocal LSM710 Upright Welcome to the Zeiss LSM 710 Upright tutorial. Before using the LSM 710