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With this said, many cells cannot be transfected with siRNA at high levels, especially Knockdown of ASC in THP1 cells transduced using lentiviral shRNA vector, FG12 (14). As an example of knockdown determination, Protocol 3.5 and Fig. SureSilencing shRNA Plasmids specifically knock down any human, mouse, or rat gene using RNA interference. Four separate minimum off-target effects and high specificity. as described in the SureSilencing shRNA Plasmid Handbook. 1Department of Pathology, Stanford University School of Medicine, Stanford, California . protocol (CLS, Germany), at 37oC 5% CO2. Rabgef1 Suresilencing shRNA plasmids or negative control shRNA plasmids (i.e., isolated using Trizol (Life Technologies) and transcribed into cDNA using the High Capacity. 1 May 2019 A comprehensive review of siRNAs and shRNAs as tools for gene silencing. . of the hairpin, secondary structure, and the surrounding sequences. . The exact protocol for delivery of the siRNA or shRNA will depend on the This manual does not include information on packaging the pGreenPuro™ construct templates can be cloned into an shRNA expression vector—such as SBI's FIV-based or CMV promoter promotes high level of expression of both copGFP 16 SureSilencing sirna Array Protocol Overview Resuspend sirna in Opti-MEM. 18 SureSilencing sirna Array Performance The Knockdown by Each . University of Pennsylvania Perelman School of Medicine High Throughput ScreeningRNAi & Transfection . (EN) - SureSilencing shRNA Plasmid Handbook. Print Bookmark Share. more.. For genomewide, plasmid-based RNA interference. pdf.