For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection. pdf. 354KB. English. Format. File size. Language. Download · Get Adobe QuantiTect Primer Assay ?????????????????????????????????????? .. QuantiTect Primer Assays?SYBR Green?????1???????2????qRT-PCR???? . Show all. Show less. Kit Handbooks. 2 For use in 2-step and 1-step qRT-PCR with SYBR Green detection for gene expression analysis. Troubleshooting Guide QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based (EN) - QuantiTect Primer Assay Handbook. Print Bookmark Share · more. QuantiTect SYBR Green RT-PCR Kit · QuantiTect Primer Assays · QuantiTect SYBR If using QuantiTect Primer Assays, the final concentration in the reaction should be 1x. Also QuantiFast SYBR Green RT-PCR Handbook 07/2011. 13. Table 2. N/A. Rhodamine. Pharmingen. Table 2. Antibodies for immunofluorescence staining .. recommended in the QuantiTect Primer Assay handbook (see Table 7). can be stored at 2–8°C or –20°C without loss of SYBR Green I fluorescence activity. PCR Kit, follow the protocols in the QuantiTect Primer Assay Handbook, Master Mix can be stored at 2–8°C or –20°C without loss of SYBR Green I RT-PCR Kit, follow the protocols in the QuantiTect Primer Assay Handbook, which To reconstitute a tube of 10x QuantiTect Primer Assay, briefly centrifuge the .. QuantiTect Primer Assay Handbook 07/2011. 15. Table 2. Reaction setup for fast PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating 2 Choose design: qPCR–2 primers–intercalating dyes