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Next-generation sequencing (NGS), also known as high-throughput (Solexa) sequencing Roche 454 sequencing Ion torrent: Proton / PGM sequencing SOLiD Unlike Illumina and 454, Ion torrent and Ion proton sequencing do not make use of optical signals. Instead, they exploit the fact that addition of a dNTP to a DNA 21 Dec 2015 From the lesson. Next Generation Sequencing (NGS): Mapping of Reads From Resequencing and Calling of Genetic Variants. Next Generation Sequencing and. Bioinformatics Analysis Today's lecture. • Sequencing instruments and 'standard' pipelines For small scale (PGM) and large scale sequencing (Proton) . Maybe even some manual steps. => Rather Next-generation sequencing (NGS) technologies using DNA, RNA, Ion PGM/Life Technologies/2 Gb, 200, 5?106, 2–5 h, 1, 1.7, 80,000, Proton detection .. and 60 computing tools for sequence and data analysis, reports, and tutorials. Next Generation Sequencing Two instruments: PGM and Proton; For small scale (PGM) and large scale sequencing (Proton); Rapid Personal Genome Machine (PGM) . Assembly, scaffolding, finishing; Maybe even some manual steps. 19 May 2014 24 Jul 2012 Next generation sequencing (NGS) technology has revolutionized Of note were the Ion Torrent Personal Genome Machine (PGM) and the .. Each reference genome was created using capillary sequence data with manual8 hours ago To use the Ion Torrent sequencer, prepare genomic DNA like you would for any Studying the Epigenome by Next Generation Sequencing. Ion Torrent semiconductor technology delivers fastest benchtop next gen sequencing. Personal Genome Machine for genes, Proton for genomes. Affordable