Eppendorf Multiporator with cell fusion module and Helix fusion chamber. Application Efficient and gentle transfer of siRNA. ‡ Electroporation of bacteria Basis-Applikations-Anleitung · Basic Applications Manual . The Eppendorf Multiporator® and the special electroporation buffers form a system which allows electroporation to take .. Transfer the cell suspension to electroporation cuvettes. The Multiporator® is designed for the electroporation of eukaryotic cells. Applications include the transfection of animal and human cell lines, embryonic stem >1 ? 1010 cfu/?g DNA using 10 pg of pUC19 and an Eppendorf Multiporator with setting of 2.5 KV Transfer the desired amount of DNA to 50 ?l of chilled cells.18 Aug 2000 Eppendorf Multiporator User Manual • 4 device description • Eppendorf Equipment. Transfer Substance. Concentration. Buffer. Temperature The efficient transfer of siRNA into cells is a prerequisite for a successful gene silencing approach. The Eppendorf Multiporator is an electroporation device manual contains information on all possible uses of the Multiporator. ® . Transfer. If the device is passed on to someone else, please include the instruction 5 ms time constant; Mainly for delivery of plasmid DNA, but also RNA, proteins and other small molecules. Technical Data; Certificates; Manuals; Notes & Papers Basis-Applikations-Anleitung · Basic Applications Manual The Eppendorf Multiporator. ® . in breeding research for nuclear transfer (the transfer of nuclei into