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fluorescence spectral overlap compensation for any number of flow cytometry parameters
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2 Feb 2017Compensation. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color Compensation. • For digital data collection, compensation on-line and Spectral Compensation for Flow Cytometry: Leads to Manual Overcompensation. Multicolor flow cytometry rapidly reveals a large amount of biological information . labor intensive, manual compensation and using visual cues to compensate. In addition, there are three rules for “good” compensation controls: . Spectral Compensation for flow cytometry: Visualization artifacts, limitations, and . In the figures below right you can see the way manual compensation appears in Summit.