FRACTIONATION OF BIOACTIVE BOTANICAL INGREDIENTS UNDER THE DIRECTION OF BIOACTIVITY ASSAYS Jian Zhang, Jianping Ye, and Zhijun Liu. 2005. Bioassay-guided fractionation of the Rubus suavissimus leaf extracts possessing NF-Kappa-B inhibitory activities and a separable cytotoxicity

Materials and methods: Extracts were obtained by maceration of all parts of plant, and subjected to bioassay-guided fractionation of methanol extract by partition column chroma-tography. The major chemical groups, saponins, reducing sugars, alkaloids, steroids, triterpen- Isolation of an Antimicrobial Compound from Impatiens balsamina L. using Bioassay-Guided Fractionation Xiaolong Yang,1 D. Kristen Summerhurst,1 Susan F. Koval,2 Christine Ficker,3 Myron L. Smith3 and Mark A. Bernards1* 1Department of Plant Sciences, University of Western Ontario, London, ON, Canada 2Department of Microbiology and Immunology, University of Western Ontario, London, ON, Canada The aim of this work was to develop an original SPE-based method to select and effectively fractionate marine natural extracts in order to obtain in one step a rough separation of the main major chemical classes. The analytical protocol was also designed to be integrated in traditional procedures of high-throughput bioassay guided fractionation. Bioassay-guided Fractionation, Antihyperglycemic and Antioxidant Properties of the Methanol Leaf Extract of Helianthus annuus *Onoja S. O.1 and Anaga A. O.2 1Department of Veterinary physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike. present study was undertaken to in part identify the potent antihyperglycemic fraction from the aqueous extract of the plant, using bioassay guided fractionation. Methods: Aqueous extract of C the bioassay guided fractionation and isolation of Lupenol and its ?-amylase inhibitory activity for the first time for this plant. II. Materials and Methods