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13 Feb 2003 1260 Infinity IsoPump/QuatPump User Manual. Notices. © Agilent . Installation Information on Leak and Waste Handling 41. Installing the Pump 45 Cleaning the Module 141. Checking and HPLC System Tool Kit 181. Guide to LC Columns and The pump – the heart of an LC system. 13 Cleaning a reversed-phase polymeric column 59 interest. Chromatographers around the world are using HPLC techniques to ensure the safety of our food and water,. 25 Feb 2010 injection systems: manual injector and autosampler. This chapter provides information for upgrading the LC system. Algae Growth in HPLC Systems 316 .. Typically < 0.1 %, < 0.05 % with external needle cleaning. HPLC System Components . Tip: Column washing eliminates the peak splitting, which resulted from a contaminant Split Peaks from Injection Solvent Effects.Use only HPLC-grade solvents and water filtered through 0.2 µm filters. • Residues or Flush the column with appropriate solvent found in the column manual. . Do not refill seal wash solvent, always use properly cleaned, fresh bottles.