However, the LFA detection restrict for proteins is inferior to lab-based assays, such as the enzyme-linked immunosorbent assay , and must be improved. Aiming to improve the protein sensitivity of the LFA, we employed an aqueous two-phase micellar system composed of Triton X-114 surfactant to concentrate Tf previous to the detection step. However, one problem with concentrating small biomolecules, similar to proteins, is that they partition evenly between the 2 phases because of experiencing…